Data/hora: 21/05/2024 23:50:52

Biblioteca(s): 	Embrapa Pecuária Sudeste.	
Data corrente: 	08/03/2021	
Data da última atualização: 	26/04/2021	
Autoria: 	LIMA, C. S.; PEREIRA, M. H.; ALEMÁN GAINZA, Y.; HOSTE, H.; REGASINI, L. O.; CHAGAS, A. C. de S.	
Afiliação: 	CAROLINE SPRENGEL LIMA, UNESP; MATHEUS HENRIQUE PEREIRA, UNESP; YOUSMEL ALEMÁN GAINZA, CNPq; HERVÉ HOSTE, INRA; LUÍS OCTAVIO REGASINI, UNESP; ANA CAROLINA DE SOUZA CHAGAS, CPPSE.	
Título: 	Anthelmintic effect of Pterogyne nitens (Fabaceae) on eggs and larvae of Haemonchus contortus: analyses of structure-activity relationships based on phenolic compounds.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Industrial Crops and Products, v.164, june 2021, 113348.	
Páginas: 	8 p.	
Idioma: 	Inglês	
Conteúdo: 	Due to high prevalence and large pathogenicity, Haemonchus contortus is the main gastrointestinal nematode in tropical and subtropical regions. This species is responsible for severe economic losses to sheep and goat breeders in Brazil. The control of this parasite is currently compromised, mainly, due to anthelmintic resistance. In the search for natural anthelmintic alternatives, Pterogyne nitens, a native Brazilian tree with potential ethnopharmacological activity, has been identified. The aim of this study was to evaluate the anthelmintic activity of ethanolic extracts and phenolic compounds from P. nitens, as well as two commercial flavonoids (chrysin and morin), to derive the chemical structure and anthelmintic activity. The ovicidal and larvicidal activity of ethanolic extracts from leaves (EEL) and fruits (EEFR), as well as natural compounds from P. nitens on H. contortus were evaluated through egg hatch assay (EHA) and larval development assay (LDA). The results showed that all extracts, especially the phenolic compounds were active in the EHA and LDA. The egg hatch inhibitory effects of EEL (EC50 = 316 &#956;g/mL) were more potent than EEFR (EC50 = 512 &#956;g/mL). However, larval development inhibitory effects of EEL (EC50 = 47 &#956;g/mL) and EEFR (EC50 = 35 &#956;g/mL) were similar. Among the compounds, the flavones (sorbifolin, pedalitin, and chrysin) did not have inhibitory effects on egg hatching but presented some activity against larval development of H. contortus. In contrast, the flavonols (quercetin, rutin, and morin) showed high activity in the EHA but were inactive in the LDA. The addition of at hydroxyl group and rutinose group to the flavonoid structure increased the ovicidal and larvicidal activity, respectively. The phenolic acids showed potent anthelmintic activity: caffeic acid, ferulic acid, and gallic acid had the highest anthelmintic effects, presenting EC50 values of 1.48, 0.56, and 4.93 &#956;g/mL in the EHA; and 31, 22, and 33 &#956;g/mL in the LDA, respectively. These results suggest that P. nitens might be a source of effective alternative compounds to control H. contortus.	
Palavras-Chave: 	Egg hatch assay (EHA); Ethanolic extracts; In vitro; Larval development assay (LDA).	
Thesaurus Nal: 	Anthelmintics; Phenolic compounds.	
Categoria do assunto: 	H Saúde e Patologia	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/221715/1/AnthelminticEffectPterogyne.pdf	
Registro original: 	Embrapa Pecuária Sudeste (CPPSE)	
Exemplares: 	CPPSE - (25258-1) - UPC - AP - DD - PROCI-2021.00007 - LIM - 2021.00007 - DIS